azdye546 azide Search Results


azdye546 picolyl azide  (Jena Bioscience)
94
Jena Bioscience azdye546 picolyl azide
Azdye546 Picolyl Azide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/azdye546+azide/AZDye546-Picolyl-Azide/bio_rxiv__64898__2026__04__29__721357-209-22-23
Average 94 stars, based on 1 article reviews
azdye546 picolyl azide - by Bioz Stars, 2026-09
94/100 stars
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azdye546 azide  (Jena Bioscience)
94
Jena Bioscience azdye546 azide
HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and <t>AZDye546-azide,</t> 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.
Azdye546 Azide, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/azdye546+azide/AZDye546-Azide/bio_rxiv__64898__2026__04__29__721357-209-9-11
Average 94 stars, based on 1 article reviews
azdye546 azide - by Bioz Stars, 2026-09
94/100 stars
  Buy from Supplier

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HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and AZDye546-azide, 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.

Journal: bioRxiv

Article Title: Chlamydia trachomatis deploys sphingolipids for genome organisation

doi: 10.64898/2026.04.29.721357

Figure Lengend Snippet: HeLa 229 WT (a), HeLa WT (e, f) or HeLa CERT K.O.(e, f) cells were infected with C. trachomatis in presence (f) or absence (a-e) of TFSM2 for the indicated duration. Then either RNA was extracted and expression of host cell SMases (SMPD1-SMPD4) were measured by RT-qPCR (a, b), cells were lysed and cellular neutral sphingomyelinase (NSM, c) as well as acid sphingomyelinase (ASM, d) activity was determined or samples were fixed, immunostained for chlamydial HSP60, CERT and DNA (e). Scale bar: 50 µm. Samples incubated with TFSM2 (e), were stained with BODIPY-FL-DBCO and AZDye546-azide, 4-fold expanded and FRET efficiencies in inclusions and host cell areas were determined. FRET efficiency ratios “inclusion vs. host cell” were calculated. n≥3. Statistics: Two-way RM ANOVA and Šidák’s multiple comparison.

Article Snippet: Subsequently, alkyne groups of TFSM derivatives were stained with AZDye546 azide [Jena Bioscience, Cat. No. CLK-1283; product discontinued and we recommend using AZDye546-picolyl-azide (Jena Bioscience, Cat. No. CLK-1284) alternatively] for 1 h/37°C at 170 rpm.

Techniques: Infection, Expressing, Quantitative RT-PCR, Activity Assay, Incubation, Staining, Comparison